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Figure 3 | BMC Cancer

Figure 3

From: Cross-talk between alpha1D-adrenoceptors and transient receptor potential vanilloid type 1 triggers prostate cancer cell proliferation

Figure 3

The α 1D -AR and TRPV1 cross-talk in PC3 cells. (A) The measurement of the extracellular acidification rate using an eight-channel Cytosensor TM microphysiometer was performed with PC3 cells stimulated for 30 sec with vehicle (control) or NA (100 μM) alone or in combination with WS433 (1 μM) or/and CPZ (1 μM). Data shown are the mean ± SD of three separate experiments. Statistical analysis was performed by comparing NA-treated cells with control cells (*) and NA + WS433-, NA + CPZ- and NA + WS433 + CPZ- with NA-treated cells (**), p ≤ 0.01. (B) The time course of [Ca2+]i increase in Fluo-3-loaded PC3 cells vehicle-treated (control) or treated with NA alone or in combination with WS433 or/and CPZ was evaluated by FACS analysis. Data shown are the mean ± SD of three separate experiments. Statistical analysis was performed by comparing NA-treated cells with control cells (*) and NA + WS433-, NA + CPZ- and NA + WS433 + CPZ- with NA-treated cells (**), p ≤ 0.01.

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