-AR and TRPV1 in PCa specimens. (A-B) Fold expression levels of α1D-AR and TRPV1 in PCa and BPH specimens analysed by qRT-PCR. Each point represents the expression level in a single subject. (C) Correlation between the α1D-AR and TRPV1 expression levels. Spearman’s rank correlation coefficient (r) is indicated in the graph. (D-E) Fold expression levels of α1D-AR and TRPV1 in PCa and bicalutamide-treated PCa specimens analysed by qRT-PCR. Each point represents the expression level in a single subject. The horizontal bars within the plots indicate the median percentage for each group. Significant differences between groups were identified by unpaired t tests and are indicated above the groups: *p < 0.0001. (F) Fluorescence microscopy analysis was performed in PCa and BHP tissues double-stained with anti-TRPV1 and anti-α1D-AR Abs, followed by Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary Abs, respectively. Magnification: 10×. Data shown are representative of one out of three separate experiments.