Figure 8From: Human hematopoietic signal peptide-containing secreted 1 (hHSS1) modulates genes and pathways in glioma: implications for the regulation of tumorigenicity and angiogenesis Purified h HSS1 inhibits HUVEC tube formation in a concentration-related manner. HUVEC growing on top of matrigel were treated with different concentrations of purified hHSS1 or vehicle control (PBS). Cells were pre-treated with hHSS1-His protein or vehicle control for 3Â h before plating on top of matrigel. After 8Â h, cells were stained with crystal violet and tube formation was evaluated. Images (100x, magnification) are representative of two independent experiments done in duplicate. A and C) Inhibitory effect of purified hHSS1-His on tube formation using 500 nM and 200 nM of hHSS1 protein, respectively. B and D) Vehicle control was diluted following the protein dilution scheme.Back to article page