Effects of siRNA-mediated knockdown of LIS1 on CCA cell proliferation, invasion, and migration. A. Western blot was used to detect the expression of LIS1, AKT, p-AKT, and MMP2 in HCCC-9810 and CCLP1 cells transfected with indicated siRNAs (20 nmol/L) 48 h after transfection. B and C. The cell proliferation curve of HCCC-9810 and CCLP1 cells transfected with LIS1 siRNA or control for 48 h. D. Representative results of invasion assays. The bar graph showed quantification of cell invasion between cells transfected with LIS1 siRNA and control. E. The mRNA levels of LIS1 were detected in CCA tumor tissues or matched adjacent tissue by q-PCR.GAPDH was used as a internal control. F. Expression of miR-144 was inversely correlated with LIS1 in CCA tissues. Statistical analysis was performed using Pearson’s correlation coefficient. (R2 = 0.49, P <0.01). *P < 0.05, **P <0.01.