Figure 3

LIS1 is a new target regulated by miR-144. A. Schematic of the two predicted seed regions in the 3′-UTR of LIS1 and mutated 3′-UTR. B. Luciferase activity was assayed in HEK293T cells. The wild-type (WT) or mutated LIS1 3′-UTR reporter gene vector was co-transfected with vector or miR-144 (MUT1 and MUT2: mutations of each binding site; MUT: mutation of both binding sites). All values represent the mean of five independent experiments performed in duplicate. C. q-PCR analysis examined the mRNA level of LIS1 after overexpression of miR-144 in HCCC-9810 and CCLP1 cells. D. Endogenous protein levels of LIS1 in HCCC-9810 and CCLP1 cells after indicated treatments were detected by western blots. E. Western blot showed the expression of LIS1 in HuCTT1 and RBE cells transfected with negative control oligonucleotide (NC) or miR-144 inhibitor (anti- miR-144). Data are shown as mean ± SD and were representative of three independent experiments. *P <0.05, **P < 0.01.