MiR-144 suppressed proliferation and invasion of cholangiocarcinoma cell lines through the AKT Pathway. A. Relative miR-144 expression level in HCCC-9810 and CCLP1 cells transfected with the control (empty vector) or miR-144-expressing vector (miR-144). B and C. Effects of stably expressing miR-144 on CCA cell proliferation were determined by cell proliferation assay. D. Representative images of invasion assays. The bar graph showed quantification of cell invasion between the miR-144-transfected cells and the control cells. E. Representative results of wound healing assays. F. Quantification of migrated cells with indicated treatment. G. Western blot analysis to assess the protein levels of AKT, p-AKT, and MMP2 in HCCC-9810 and CCLP1 cells with miR-144 overexpression. GAPDH was used as an internal control to normalize protein signal intensity. Data are shown as mean ± SD and were representative of three independent experiments. *P <0.05, **P < 0.01.