Visualization of lipid bodies in LNCaP cells.
(A) Widefield fluorescent images of LNCaP and PBMC cells stained with Bodipy (green) for lipid bodies and Hoechst (blue) for nuclei. (B) CARS (red) images of lipid bodies and two-photon fluorescence (yellow, green) images of immunofluorescently labeled LNCaP cells (yellow) and PBMC cells (green). LNCaP cells were identified with TRITC conjugated primary antibodies against cytokeratin (CK). PBMC cells were identified with FITC conjugated primary antibodies against CD45.