Comparison of glucose and lipid uptake between RWPE1, LNCaP and PBMC.
(A) Glucose and (B) lipid uptake of LNCaP, RWPE1 and PBMC cells. Blue lines represent control cells without incubation with neither fluorescent glucose analog nor DiD. Red lines represent cells incubated with either fluorescent glucose analog (A) or DiD (B). Dashed lines with bar heads indicate the cut-off fluorescence intensity values that separate stained cells from unstained cells. Cut-off values were chosen to exclude >95% of control unstained cells. Cells were incubated with plasma for 16 hours, then with fluorescent glucose analog or with lipophilic DiD dye for 15 minutes. Data presented are of one set of flow cytometry experiment. (C) and (D) are graphical presentation of average flow cytometry data of at least 4 repeats per experimental condition for glucose and lipid uptake, respectively. Error bars are standard deviation across 4 repeated measurements. Asterisk indicates P-value < 0.05 determined with paired Student’s t-test against untreated control.