Figure 5
From: MicroRNA-1269 promotes proliferation in human hepatocellular carcinoma via downregulation of FOXO1
MiR-1269 promotes proliferation of HCC cells by suppressing FOXO1. A. Western blotting analysis of FOXO1 in the indicated cells transfected with FOXO1-siRNA. α-Tubulin served as the loading control. B. The effects of FOXO1 on miR-1269 mediated HCC proliferation analyzed by MTT assay. C. The quantification of crystal violet stained colonies formed by the indicated cells transfected with FOXO1-siRNA or NC. D. Quantification of colony numbers of indicated cells treated with FOXO1-siRNA or NC, which determined by anchorage-independent growth assay. Error bars represent the mean ± SD from three independent experiments. * P < 0.05.