Figure 3
ACM alters mitochondrial energy metabolism. (A) Intracellular levels of ATP were significantly reduced in OE33 cells treated with ACM from both non-obese (n = 2) and obese (n = 4) EAC patients, when compared to controls. (B) Basal OCR was significantly reduced in OE33 cells treated with ACM from non-obese EAC patients, when compared to controls. (C) Basal ECAR was significantly reduced in OE33 cells incubated with ACM from non-obese EAC patients, whilst ECAR was significantly increased in OE33 cells treated with ACM from obese patients, when compared to controls. (D) Treatment with 2-deoxyglucose (55 mM), significantly reduced ECAR levels in OE33 cells treated with ACM from obese EAC patients, when compared to controls. Data are presented as the mean ± SEM. Analysis was performed by two-tailed Student’s t-test, **p < 0.01, *p < 0.05.