Apoptosis of LV-E2F1-GFP cells and tumors in nude mice
. A: mRNA expression level of E2F1 was determined by semiquantitative reverse-transcriptase polymerase chain reaction; B: E2F1 protein expression was determined by western blotting; C: Relative tumor volume (RTV) of nude mice in each group is presented. Each time point represents the mean RTV for each group. D: Tumor cells were evaluated by HE staining and TUNEL assay (× 400); E: Percentage of apoptotic cells was analyzed by TUNEL assay. All values are mean ± SE. *
P < 0.05 for E2F1 group versus GFP group and NC group.