promoter hypermethylation is associated with ST6GAL1 gene silencing in an independent TCGA data set. (A)
ST6GAL1 expression in bladder tumor samples from the TCGA data portal. Red: high expression, black: mean expression and green: low expression. Left panel: sample type (dark grey: primary tumor; white: solid normal tissues). Right panel: ST6GAL1 mRNA expression. (B) DNA methylation of the ST6GAL1 promoter analyzed in bladder cancer samples from TCGA data portal. Red: high methylation, white: mean methylation; blue: low methylation. Right panel: sample type (dark grey: primary tumor; white: solid normal tissues). Left panels: values of ST6GAL1 DNA methylation for each available CG number. The relative positions of six analyzed CpG duplets within the ST6GAL1 promoter region covering the MSP analyzed region (+224 to +346) are indicated. +1: ST6GAL1 TSS. CG25372568 position in relation to the stated TSS: +272. (C) Inverse correlation of ST6GAL1 mRNA expression and its DNA methylation status in primary bladder cancer samples. ρ: Spearman correlation coefficient.