Figure 3

Effects of a 3 ^rd generation small molecule PKCδ inhibitor on human pancreatic cancer stem cell cultures. PCSC cells were grown to 80% confluence in 96-well plates and then exposed to BJE6-106 at concentrations ranging from 0.1 to 20 μM, or to rottlerin at concentrations ranging from 1 to 20 μM. The corresponding equivalent volume of solvent (DMSO) was used as a vehicle control (Vehicle). After 48 and 72 hr of exposure, cell mass was evaluated by MTT assay. Control values were normalized to 100%. Error bars represent SEM. p values for comparison between vehicle and rottlerin effects on cell number at 48 hr reached significance at 1 μM, and for BJE6-106 at 0.1 μM (p ≤ 0.02), and remained significant at the 72 hr time point.