Figure 3

Loss of host CD73 did not alter intratumoral immune response of B16-F10 tumors. Subcutaneously injected B16-F10 tumors, reported on in Figure 2, were excised on day 18 post injection, minced and enzymatically separated into single cell suspension. Immune cell subsets were measured with FACS. Representative dot plots of CD4⁺ T helper (A), CD8⁺ cytotoxic T cells (B), CD4⁺ CD25⁺ T cells (C), CD11b⁺ (D) and CD11c⁺ myeloid cells (E) are shown (gated cells are marked in red). (F) No differences in immune cell composition of tumors from WT and CD73^−/− mice were obtained. Data are shown as mean ± SEM (n = 4).