Overexpression of URG4 mRNA and protein in cervical cancer tissues. (a) Representative images of western blotting analyses of URG4 protein expression in eight matched pairs of cervical cancer tissues (T) and adjacent noncancerous tissues (ANT). α-Tubulin was used as the loading control. (b) The average T/ANT ratios of URG4 mRNA expression in the paired cervical cancer (T) and adjacent noncancerous tissues (ANT) were quantified using qPCR and normalised against GAPDH. The error bars represent the standard deviation of the mean (SD), which was calculated from three parallel experiments. (c) Immunohistochemical analysis of URG4 protein expression in eight pairs of matched cervical cancer tissues. *P < 0.05. **P < 0.01.