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Figure 5 | BMC Cancer

Figure 5

From: Deep Sequencing the microRNA profile in rhabdomyosarcoma reveals down-regulation of miR-378 family members

Figure 5

miR-378a-3p response to 5-aza-dC treatment. (A) Schematic representation of the miR-378a-3p promoter region generated by EMBOSS software. Bioinformatic analysis showed the presence of a high percentage of GC nucleotides and an enriched zone of CpG islands. (B) Effect of increasing doses of 5-aza-dC (2-10-20 μM) on mature miR-378-3p levels compared with control RH30 cells 24 h after treatment. Results are means ± SD of three separate experiments (**, p < 0.01; ***p < 0.001). (C) BISMA diagrams showing the methylation level of CpG dinucleotides in the miR-378a promoter in 5-aza-dC and mocked control cells, as revealed by bisulfite sequencing of BS1 and BS2 regions. Blue rectangles indicate non-methylated CpGs. (D) Methylation analysis of miR-378a promoter region by PCR using primer set for methylated (M) or unmethylated (UM) sequences. The presence of a visible UM product indicates that CpG dinucleotides are not methylated in both Ctr and 5-aza-dC-treated cells.

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