Figure 2

miR-378a-3p negatively regulates IGF1R. (A) Schematic network representation of miR-378a-3p target genes functional annotations and interactions obtained from Cytoscape Reactome FI interrogation. IGF1R represents the principal mRNA target of miR-378a-3p. Putative mRNA targets of miR-378a-3p are predicted to be involved in several signal pathways, specifically apoptosis, cell cycle and chromatin remodelling. Arrow (edge) orientation indicates positive, negative or undetermined functional interaction between different genes (network nodes). Target gene colours indicate different functional modules of the interaction network. (B) Immunoblot analysis. Validation of miR-378a-3p direct participation in the post-transcriptional regulation of IGF1R in RH30 cells (miR-control vs. miR-378a-3p positive cells). Tubulin was used as loading control. (C) Sequence alignment of miR-378a-3p seed sequence (highlighted) with IGF1R 3′-UTR binding sites (bold letters in the yellow box; position 5589–5596 of human IGF1R: NM_000875) in different species. Data from TargetScan Human 6.2 interrogation.