Everolimus decreases the Cho/H
O ratio of RIF-1 tumours. C3H mice bearing RIF-1 tumours were treated with everolimus (10 mg/kg/day) or vehicle for 5 days and the ratio of total choline to (unsuppressed) water (Cho/H2O) in tumours was measured on day 0 and at the endpoint day 5. Panel A shows a 1H-MRS spectrum from a representative RIF-1 tumour before and after treatment with everolimus. Graphs show the individual values for each tumour, n = 7 per treatment group (B) and the mean ± SEM fractional change ΔCho/H2O for each treatment (C), where *p < 0.05 as shown.