Figure 4
The effect of miR-141-mediated downregulation of HNF-3β on cell invasion and apoptosis. (A) Transwell analysis of HepG2 cells treated with equal doses of pre-miR-control, pre-miR-141, scrambled control siRNA, HNF-3β siRNA, scrambled control plasmid, HNF-3β overexpression plasmid or pre-miR-203 plus the HNF-3β overexpression plasmid. The experiment was repeated three times, and the quantitative analysis is shown in the right panel. (B) HepG2 cells were transfected with equal doses of pre-miR-control, pre-miR-141, scrambled control siRNA, HNF-3β siRNA, scrambled control plasmid, HNF-3β overexpression plasmid or pre-miR-203 plus the HNF-3β overexpression plasmid. Apoptosis profiles were analyzed by flow cytometry, and the quantitative analysis is shown in the right panel (mean ± S.D.; ** p < 0.01; *** p < 0.001).