Figure 3
The effect of miR-141-mediated downregulation of HNF-3β on cell proliferation. (A) Quantitative RT-PCR analysis of HNF-3β mRNA levels in HepG2 cells when transfected with control or HNF-3β siRNA. (B) Western blot analysis of the endogenous HNF-3β protein level in HepG2 cells when transfected with control or HNF-3β siRNA. Left: representative image; right: quantitative analysis. (C) Cell proliferation assays were performed 12, 24, 36 and 48 h after transfection of HepG2 cells with scrambled control siRNA or HNF-3β siRNA. (D) Cell proliferation assays were performed 12, 24, 36 and 48 h after transfection of HepG2 cells with pre-miR-141 or pre-miR-control. (E) Quantitative RT-PCR analysis of the HNF-3β mRNA level in HepG2 cells transfected with the control or HNF-3β plasmid. (F) Western blot analysis of the HNF-3β protein level in HepG2 cells transfected with control or HNF-3β plasmid. Left: representative image; right: quantitative analysis. (G) Cell proliferation assays were performed 12, 24, 36 and 48 h after transfection of HepG2 cells with control or HNF-3β overexpression plasmid. (H) Cell proliferation assays were performed 12, 24, 36 and 48 h after transfection of HepG2 cells with pre-miR-141, HNF-3β plasmid, or pre-miR-141 and the HNF-3β plasmid (mean ± S.D.; ** p < 0.01; *** p < 0.001).