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Figure 4 | BMC Cancer

Figure 4

From: MTDH mediates trastuzumab resistance in HER2 positive breast cancer by decreasing PTEN expression through an NFκB-dependent pathway

Figure 4

MTDH modulated PTEN expression via NF-κB signaling pathway. A. MTDH downregulation resulted in increased IκBαexpression and decreased p65 nuclear translocation in SK-BR-3/R cells. High level of PTEN and decreased p-Akt were also observed. MTDH overexpression activated NF-κB by promoting IκBα degradation and p65 nuclear translocation in SK-BR-3 cells. Decreased PTEN expression and high level of p-Akt were observed. β-actin and lamin B. were subcellular loading controls for cytoplasmic and nuclear extracts respectively; B. Immunofluorescence imagings of p65 in cells with MTDH manipulation (magnification at 400×); C. Relative PTEN-Luc activity after co-transfection of p65 and MTDH-shRNA in SK-BR-3/R cells (*P < 0.05); D. Relative PTEN-Luc activity after co-transfection of p65 and MTDH in SK-BR-3 (*P < 0.05).

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BMC Cancer

ISSN: 1471-2407

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