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Table 1 Assays selected and PCR conditions for Q-RT-PCR of mRNA analysis

From: Validation of COL11A1/procollagen 11A1 expression in TGF-β1-activated immortalised human mesenchymal cells and in stromal cells of human colon adenocarcinoma

Gene

Assay ID

COL11A1

Hs01097664_m1

GAPDH

Hs02758991_g1

PUM1

Hs004472881_m1

RPL10

Hs00749196_s1

DES

Hs00157258_m1

ACTA2

Hs00426835_g1

VIM

Hs00185584_m1

  1. PCR conditions were: 50°C – 2 min; 95°C – 10 min; and 40 amplification cycles:
  2. 95°C – 15 sec and 60°C – 1 min.
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BMC Cancer

ISSN: 1471-2407

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