Figure 4

Involvement of STATIP1 and STAT3 genes in IM resistance in CML cell lines. (A) STATIP1 mRNA levels in K562 and Lucena cells determined by RT-qPCR. (B) STAT3 mRNA levels, as determined by RT-qPCR, in Lucena cells under the following conditions: 1 μM IM treatment, 40 μM LLL-3 treatment, and co-treatment after 24 h. (C) The ABCB1 mRNA levels in Lucena cells were determined by RT-qPCR under the treatment conditions noted above. (D) Apoptotic cells were measured by flow cytometry in both cell lines under the treatment conditions noted above. The cell cycle was evaluated by flow cytometry after being subjected to the treatment conditions noted above in K562 (E) and Lucena (F) cells. All comparisons were made to untreated cells – ctrl. Ctrl: control. The data represent the mean ± SD of at least three independent experiments (*p <0.05 and **p <0.01).