Figure 4

Effect of Notch inhibition during pancreatitis-induced PanIN formation – Preliminary data. (A) Age-matched wild type (control), p48Cre;LSL-Kras ^G12D (KC), and p48Cre;LSL-Kras ^G12D ; Rosa ^DNMAML-GFP/+ (KC;DNMAML) mice were administered intraperitoneal injections of caerulein, a cholecystokinin agonist, over the course of 48 hours. Pancreata was harvested and analyzed for PanIN development. (n = 2 mice/time point). (B) H&E staining of caerulein-treated wild type (control), p48Cre;LSL-Kras ^G12D (KC), and p48Cre;LSL-Kras ^G12D ; Rosa ^DNMAML-GFP/+ (KC;DNMAML) pancreata at high magnification (20x). (n = 2 mice/time point). (C) Quantification of pancreatic intraepithelial neoplasia (PanIN) at the indicated time points of age-matched p48Cre;LSL-Kras ^G12D (KC), and p48Cre;LSL-Kras ^G12D ; Rosa ^DNMAML-GFP/+ (KC;DNMAML) mice (n = 2 mice/genotype). Color coding as follows: acini (dark purple), acinar-ductal metaplasia (ADM; green), PanIN1A (light blue), PanIN1B (blue), PanIN2 (red), PanIN3 (dark red). Data represents mean ± SEM. n.s.: not statistically significant.