Figure 6From: Constitutive activation of the ERK pathway in melanoma and skin melanocytes in Grey horses Activation of ERK pathway in skin melanocytes of Grey horses. (A) Double immunofluorescence staining was performed for P-ERK1/2 (green in a1-a2, c1-c2) or ERK1/2 (green in b1-b2, d1-d2) and MITF (red in a2, b2, c2, d2) in skin tissue sections from non-grey and Grey horses; a3, b3, c3, d3, bright field images of the sections. Dotted lines, skin basal membrane; e, epidermis; d, dermis. Scale bar, 10 μm. Note an increase in P-ERK1/2 signal (arrow heads in c1-c2) in the surrounding keratinocytes in Grey horse skin. (B) Quantification of P-ERK1/2 and ERK1/2 immunofluorescent signals in relation to the respective total number of MITF+ cells in skin tissue sections from non-grey (n =9; dark grey bars) and Grey (n =12; light grey bars) horses shown as the mean ± s.e. of three independent experiments. ***P <0.001.Back to article page