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Figure 5 | BMC Cancer

Figure 5

From: Selective inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86

Figure 5

SPP86 inhibits RET- mediated proliferation. (A) Estrogen- deprived and serum starved MCF7 cells were left untreated or exposed to 1 ng/ml E2 and/or 10 ng/ml GDNF alone or in combination with 1 μg/ml SPP86 in phenol red- free media for 7 days. Proliferation was expressed as fold increase in growth relative the untreated control population. The data represent the mean of 3 experiments ± S. E; *p <0.05 treated vs. untreated for each series. (B) Estrogen- deprived and serum starved MCF7 cells were cultured in the presence of 1 nM E2 (estrogen) together with 10 ng/ml of GDNF or 10 ng/ml insulin in the presence or absence of 1 μM SPP86 for 72 h. Proliferation was expressed as fold increase in growth relative the untreated control population. The data represent the mean of 3 experiments ± S. E; *p <0.05. (C) Estrogen- deprived and serum starved MCF7 cells exposed to increasing concentrations of SPP86 or 4-OHT in phenol red- free media containing 1 ng/ml E2 and 10 ng/ml GDNF for 7 days. Viability was expressed as a percentage of the untreated control population. The data in each panel represent the mean of 3 experiments ± S. E.; *p <0.05, **p <0.0001.

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