Figure 5

Of the FASTKD isoforms only FASTKD2 mediates apoptosis. Hela cells were transfected to express the 5 FASTKD isoforms as YFP chimeras with YFP at the C-terminus. Fifteen h later cells were fixed and permeablized for analysis of YFP fluorescence (green) and apoptosis by TUNEL assay (red). Nuclei were stained with DAPI (blue). The FASTKD-YFP chimeras localize in a peri-nuclear fashion which was previously documented to reflect mitochondrial localization [12]. Only expression of FASTKD2 leads to apoptosis.