Figure 4

FASTKD2 expression is enhanced by DD1 activation in LNCaP cells and is the only member of the FASTKD gene family that is enhanced by DD1. (A) Domain structure of FASTKD2. (B) LNCaP-AI cells and HeLa cells stably expressing DD1-ERT2 or DD1(S28A)-ERT2 were first incubated with 20 uM zVDVAD-fmk to block apoptosis and then treated with 4-OHT (1 uM) or EtOH vehicle for 8 h. FASTKD2 mRNA expression was examined by qRT-PCR. Fold induction represents the FASTKD2 expression values of 4-OHT treated cells relative to those of EtOH treated cells. Data represents the mean +/- SEM from three representative experiments and the p values are given in the text. (C) LNCaP-AI and T-47D breast cancer cells stably expressing DD1-ERT2 were incubated with 4-OHT (1 uM) or EtOH vehicle for 8 h (cells were pretreated with 20 uM zVDVAD-fmk to block apoptosis). The expression of the 5 FASTKD mRNAs (indicated as KD1, KD2, KD3, KD4, and KD5) was examined by qRT-PCR using the primers indicated in the Methods section and in [12]. Fold induction represents the FASTKD expression values of 4-OHT treated cells relative to those of EtOH treated cells. Data represents the mean +/- SEM from three representative experiments and the p values are given in the text.