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Figure 5 | BMC Cancer

Figure 5

From: High mobility group A1 protein expression reduces the sensitivity of colon and thyroid cancer cells to antineoplastic drugs

Figure 5

Overexpression of HMGA1 correlates with the activation of prosurvival pathway. (A) Cells were treated with 50 μM of 5-fluorouracil (left panel) or with 500 nM of doxorubicin (right panel) and collected at the indicated time points after drug treatment. Protein extracts were analyzed western blot to evaluate Akt and p70 phosphorylation. Total Akt levels are also reported. β-Actin was reported as western blot loading control. (B) Cell viability curve of GEO CR and FRO transfected with a short hairpin RNA targeting the Akt gene or with a control short hairpin RNA and, after 24 hours of transfection, treated with the indicated doses of 5FU or doxorubicin. Values are the mean ± S.D. of three experiments performed in triplicate. *p < 0.05 versus cells transfected with empty vector (GEO CR pLKO.1 or FRO pLKO.1).

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