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Figure 4 | BMC Cancer

Figure 4

From: High mobility group A1 protein expression reduces the sensitivity of colon and thyroid cancer cells to antineoplastic drugs

Figure 4

Silencing of HMGA1 triggers caspase activation in response to antineoplastic drugs. (A) Cells were treated with 50 μM of 5-fluorouracil (left panel) or with 500 nM of doxorubicin (right panel) and collected at the indicated time points after drug treatment. The uncleaved procaspase-3 was detected by Western blot analysis using a specific antibody. Data were normalized with β-actin expression. (B) Colorimetric caspase 3/7 activity assay, performed on GEO CR pLKO.1 and GEO CR shHMGA1 treated with 50 μM of 5-fluorouracil (left panel) and on FRO pLKO.1 and FRO shHMGA1(right panel) treated with 500 nM of doxorubicin and harvested 6 h after the treatment. Values are the mean ± S.D. of three experiments performed in triplicate. *p < 0.05 versus cells transfected with empty vector (GEO CR pLKO.1 or FRO pLKO.1).

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BMC Cancer

ISSN: 1471-2407

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