miR-200a regulated EMT of CSCs in pancreatic cancer. (A) MiR-200a mimic was efficient in CSCs. (B) Overexpression of miR-200a in CSCs of PANC-1 resulted in up-regulation of E-cadherin and down-regulated expression of N-cadherin, ZEB1 and vimentin as assessed by Real-Time PCR. (C-E) The morphology of CSCs changed from sphericity to epithelial-like appearance after miR-200a transfection. (C) The morphology of CSCs control, (D) CSCs transfected by control mimic and (E) CSCs transfected by miR-200a mimic (Scale bar = 50 μm). Data represent mean ± SD. * = significantly different from control, P < 0.05, ** = significantly different from control, P < 0.001. Data represent mean ± SD.