Figure 1

Presence of CD44, CD24 and ESA on cell surface in pancreatic carcinoma cell line PANC-1. Flow cytometric analysis was used to determine the presence of CD44, CD24, and ESA on the cell surface of pancreatic adenocarcinoma cell line PANC-1. (A) The presence of CD44⁺CD24⁺ population (Q2) of PANC-1 cell line. (B) The presence of CD44⁺CD24⁺ESA⁺ population (P2) of PANC-1 cell line. The morphology of PANC-1 cell line (C) and CSCs (D) (Scale bar = 50 μm). (E)The expressions of Oct4 and Nanog were measured by real-time PCR in PANC-1 and CSCs. Data represent mean ± SD. * = significantly different from PANC-1, P < 0.05, ** = significantly different from PANC-1, P < 0.001.