Table 1 Primers for site-directed mutagenesis of human TOP2B
From: Dexrazoxane may prevent doxorubicin-induced DNA damage via depleting both Topoisomerase II isoforms
T65I | forward | cattcttcttcgtcctgatatatatattgggtcagtggagc |
reverse | gctccactgacccaatatatatatcaggacgaagaagaatg | |
Y66F | forward | tcttcgtcctgatacatttattgggtcagtggagc |
reverse | gctccactgacccaataaatgtatcaggacgaaga | |
R178Q | forward | gatgagaaaaaagttacaggtggtcaaaatggttatggtgcaaaactttgta |
reverse | tacaaagttttgcaccataaccattttgaccacctgtaacttttttctcatc | |
Y181S | forward | aaagttacaggtggtcgtaatggttctggtgcaaaactttg |
reverse | caaagttttgcaccagaaccattacgaccacctgtaacttt | |
L185F | forward | ggtcgtaatggttatggtgcaaaattttgtaatattttcagtacaaagt |
reverse | actttgtactgaaaatattacaaaattttgcaccataaccattacgacc |