Figure 6

Identification of transcripts dysregulated by miR-9 overexpression in normal murine BMMCs and P815 malignant mast cells. (A) Transcriptional profiling of mBMMCs expressing pre-miR-9-3 lentivirus or empty vector control was performed using Affymetrix GeneChip® Mouse Gene 2.0 ST Arrays to identify genes showing differential expression (>2-fold) with miR-9 overexpression. Real-time PCR was performed to validate changes in gene expression for transcripts (HSPE, TLR7, PERP, PPARG, SLPI) altered by miR-9 overexpression in mBMMCs (*p < 0.05). (B) Transcriptional profiling of P815 mast cells expressing pre-miR-9-3 lentivirus or empty vector control was performed as described above. Real-time PCR was performed to independently validate expression levels of genes (SERPINF1, MLANA, CD200R1, CD200R4) altered by enforced miR-9 expression in P815 cells (*p < 0.05). (C) Mouse BMMCs and P815 cells expressing pre-miR-9-3 lentivirus or empty vector control were collected and real-time PCR for IFITM3, PDZK1IP1, and CMA1 was performed (*p < 0.05). Three independent experiments were performed using cells from 3 separate transduction experiments and all reactions were performed in triplicate.