Figure 3
MEK1 inhibition with carboplatin treatment. Silencing of MEK1 significantly increases the ratio of the apoptotic cells, and decrease the ratio of the viable cells after 48 hours carboplatin treatment compared to the negative control siRNA transfected cells. *: p < 0.05 (A) Dose–response curves of SKOV-3 and CAOV-3 cell lines against the MEK1 inhibitor PD0325901 (B). Effects of 48 hour treatment with carboplatin and PD0325901 as single agents and in combination. SKOV-3: C1: 212 μM carboplatin, C2: 141 μM carboplatin, PD: 554 nM PD0325901 in SKOV3 cell line. CAOV-3: C1: 111 μM carboplatin, C2: 74 μM carboplatin, PD: 277 nM PD0325901 in CAOV-3 cell line (mean with SEM) *p < 0.0001 C).