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Table 2 Initial and final velocities of all three cell lines on random and aligned nanofibers

From: Preferential, enhanced breast cancer cell migration on biomimetic electrospun nanofiber ‘cell highways’

Test variable

Cell type

Fiber orientation

Initial velocity (um/h) [ [t avg = 1-8 h]

Final velocity (um/h) [ [t avg=16-24 h]

Control

MCF-10A

Random

2.05 ± 1

1.81 ± 1

Aligned

10.85 ± 8

10.02 ± 4

MCF-7

Random

3.01 ± 3

4.1 ± 4

Aligned

11.06 ± 8.4

10.86 ± 5.4

MDA-MB-231

Random

10.85 ± 8

6.1 ± 10

Aligned

31.6 ± 14

29.8 ± 7

Vehicle

MCF-10A

Random

2.95 ± .85

1.61 ± 1

Aligned

11.25 ± 3

10.1 ± 3

MCF-7

Random

2.71 ± 2

3.92 ± 2

Aligned

10.36 ± 4

10.56 ± 2

MDA-MB-231

Random

10.03 ± 6

8.1 ± 2

Aligned

28.4 ± 17

25.8 ± 10

CXCL12

MCF-10A

Random

3.1 ± 5

2.0 ± 2

Aligned

11.31 ± 4

12.1 ± 5

MCF-7

Random

7.1 ± 5

6.0 ± 3

Aligned

15.26 ± 13

15.82 ± 5

MDA-MB-231

Random

11.2 ± 6.1

5.1 ± 6

Aligned

32.3 ± 13

19.3 ± 9***

  1. ***Denotes statistical significance, p = .00001.
  2. No significant difference was seen for any of the cell-lines early vs. late for the control or vehicle experimental conditions. MDA-MB-231 cells displayed a significant drop in velocity in presence of a CXCL12 gradient from early to late time points resulting in a -1.21 um/h2 deceleration, likely due to the dissipation of the gradient at later time points and inherent sensitivity of the CXCR4 receptor.