Figure 4

Effect of miR-26a mimic and siRNAs on target gene protein expression in PCa cell lines. Protein levels of (A) AMACR and (B) EZH2 were determined by Western Blot and normalized to α-tubulin. Normalized values are shown relative to the corresponding control treatments (100%): miR-CON (100 nM) for treatment with miR-26a mimic and siR-CON (150 nM) for treatment with siRNAs, respectively. Values represent averages of two to five independent experiments with their mean deviation. A one-tailed paired t-test was used to compare differences between cells treated with miR-26a mimic or siRNAs and the respective control treated cells (miR-CON or siR-CON): *p < 0.05, **p < 0.01, ^#p < 0.1 (statistical trend). (C) Exemplary Western Blots for the detection of AMACR protein following treatment with 100 nM miR-26a mimic or miR-CON are depicted. Alpha-tubulin served as loading control. Lines indicate that the sample lanes were not adjacent in the original gel. However, samples per cell line were retrieved from the same experiment and each sample was simultaneously probed for AMACR and α-tubulin.