Figure 1

Changes of tumor blood flow and tumor histology at 2, 4, 24, 48 and 72 hours after treatment with 10 μg MECA32-Fab-TF. Tumor blood flow was monitored using 3D power Doppler sonography (upper panel). The histology sections were stained with hematoxylin and eosin (lower panel). There were two mice at each time point. Results were the same between two mice at each time point. Only result from one of the two mice studied at each time point is shown. Upper panel shows change of tumor blood flow before and after treatment. White arrows point at blood flow signal in tumors. Blood flow signal disappeared at 2 hours and persisted up to 72 hours. Lower panel shows that fibrin thrombi (balck arrows) in blood vessels became evident at 2 hours after treatment and persisted throughout the study period. Tumor tissue became morphologically degenerated at 24 hours. Frank necrosis became evident at 48 hours. Photomicrographs were taken at 100x magnification.