Immunohistochemical staining of adiponectin, AdipoR1, and AdipoR2. (a–c) Localization of adiponectin staining in normal colon tissue (a, ×200), advanced adenoma (b, ×200) and CRC (c, ×200) differed from spindle-shaped cells in the subepithelial area to a diffuse area in the lamina propria. (d–i) Staining for AdipoR1 (d, ×200) and AdipoR2 (g, ×200) was positive in the epithelium of normal colon tissues. AdipoR1 (e, ×400) and AdipoR2 (h, ×100) showed moderate staining intensities in the epithelial layer of advanced adenoma. In CRC, AdipoR1 (f, ×400) and AdipoR2 (i, ×100) showed weak or no staining.