Figure 7
Biological confirmation of gene expression analysis. (A) qPCR analysis measuring the fold change of mitotic regulators following hyperthermia treatment. RQ values are represented as the hyperthermia-induced change in gene expression for each gene relative to the expression of the same gene in the normal temperature condition (RQ?=?1). The data shown are the median of at least 3 replicates, plus or minus the standard deviation, and presented in log scale. (B) The panel of mammary epithelial and breast cancer cells were grown under standard growth conditions or treated with 30 minutes fever range hyperthermic shock. Cells were harvested after 24 hours and cell cycle analysis was performed using flow cytometric detection of propidium iodide intensity.