Figure 4From: RNA-seq profiling of a radiation resistant and radiation sensitive prostate cancer cell line highlights opposing regulation of DNA repair and targets for radiosensitization Nuclear expression of BRCA1 and RAD51 following irradiation in the LNCaP and PC-3 cell lines. Nuclear proteins were extracted from cells at 0 (non-irradiated), 6 and 24Â hours following exposure to 2Â Gy irradiation. Protein expression was analysed via Western blot. HeLa nuclear protein extract was utilised as a positive control and membranes were probed for Sp1 as a loading control.Back to article page