Table 3 The primers used in PCR amplification and sequencing
Genes | Primers (sequence 5’-- > 3’) |
|---|---|
KRAS | Â |
 Exon 2 | F: TTAACCTTATGTGTGACATGTTCTAA |
| Â | R: ATCAAAGAATGGTCCTGCAC |
BRAF | Â |
 Exon 15 | F: CTTTACTTACTACACCTCAG |
| Â | R: TAACTCAGCAGCATCTCAGG |
PIK3CA | Â |
 Exon 9 | F: AGTAACAGACTAGCTAGAGACAAT |
| Â | R: CATGCTGAGATCAGCCAAAT |
 Exon 20 | F: ATGATGCTTGGCTCTGGAAT |
| Â | R: TGTGGAATCCAGAGTGAGCTT |