|  | KRAS exon 2 | BRAF exon 15 | PIK3CA exon 9&20* | PI3K/RAS-RAF pathway* | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  | No. patients (214/212) | No (%) | Yes (%) | P | No (%) | Yes (%) | P | No (%) | Yes (%) | P | No (%) | Yes (%) | P |
Sex | male | 127 (126) | 73 (61.9) | 54 (56.3) | 0.406a | 122 (59.5) | 5 (55.6) | 1.000b | 109 (58.6) | 17 (65.4) | 0.509a | 60 (61.2) | 66 (57.9) | 0.623a |
 | female | 87 (86) | 45 (38.1) | 42 (43.8) |  | 83 (40.5) | 4 (44.4) |  | 77 (41.4) | 9 (34.6) |  | 38 (38.8) | 48 (42.1) |  |
Age | Â | 68.0 | 67.1 | 69.1 | 0.286d | 68.0 | 66.9 | 0.801d | 68.7 | 64.6 | 0.133d | 67.8 | 68.4 | 0.728d |
Location | colon | 126 (124) | 73 (61.9) | 53 (55.2) | 0.325a | 117 (57.1) | 9 (100.0) | 0.011 b | 103 (55.4) | 21 (80.8) | 0.014 a | 54 (55.1) | 70 (61.4) | 0.353a |
 | rectum | 88 (88) | 45 (38.1) | 43 (44.8) |  | 88 (42.9) | 0 (0) |  | 83 (44.6) | 5 (19.2) |  | 44 (44.9) | 44 (38.6) |  |
Differentiation | well | 29 (29) | 19 (16.1) | 10 (10.4) | 0.912c | 28 (13.7) | 1 (11.1) | 0.131c | 22 (11.8) | 7 (26.9) | 0.215c | 15 (15.3) | 14 (12.3) | 0.521c |
 | moderate | 163 (161) | 83 (70.3) | 80 (83.3) |  | 159 (77.6) | 4 (44.4) |  | 145 (78.0) | 16 (61.5) |  | 73 (74.5) | 88 (77.2) |  |
 | poor | 7 (7) | 7 (5.9) | 0 (0) |  | 5 (2.4) | 2 (22.2) |  | 5 (2.7) | 2 (7.7) |  | 3 (3.1) | 4 (3.5) |  |
 | missing | 15 (15) | 9 (7.6) | 6 (6.3) |  | 13 (6.3) | 2 (22.2) |  | 14 (7.5) | 1 (3.8) |  | 7 (7.1) | 8 (7.0) |  |
Tumor diameter | <5Â cm | 103 (102) | 53 (44.9) | 50 (52.1) | 0.254c | 101 (49.3) | 2 (22.2) | 0.171b | 93 (50.0) | 9 (34.6) | 0.172a | 46 (46.9) | 56 (49.1) | 0.710a |
 | > = 5 cm | 108 (107) | 64 (54.2) | 44 (45.8) |  | 101 (49.3) | 7 (77.8) |  | 91 (48.9) | 16 (61.5) |  | 51 (52.0) | 56 (49.150.0) |  |
 | missing | 3 (3) | 1 (0.8) | 2 (2.1) |  | 3 (1.5) | 0 (0) |  | 2 (1.1) | 1 (3.8) |  | 1 (1.0) | 2 (1.8) |  |
TNM-stage | I | 32 (32) | 15 (12.7) | 17 (17.7) | 0.828c | 32 (15.6) | 0 (0) | 0.007 c | 26 (14.0) | 6 (23.1) | 0.433c | 13 (13.3) | 19 (16.7) | 0.231c |
 | II | 78 (77) | 50 (42.4) | 28 (29.2) |  | 76 (37.1) | 2 (22.2) |  | 69 (37.1) | 8 (30.8) |  | 44 (44.9) | 33 (28.9) |  |
 | III | 82 (81) | 38 (32.2) | 44 (45.8) |  | 79 (38.5) | 3 (33.3) |  | 70 (37.6) | 11 (42.3) |  | 31 (31.6) | 50 (43.9) |  |
 | IV | 19 (19) | 13 (11.0) | 6 (6.3) |  | 15 (7.3) | 4 (44,4) |  | 18 (9.7) | 1 (3.8) |  | 8 (8.2) | 11 (9.6) |  |
 | missing | 3 (3) | 2 (1.7) | 1 (1.0) |  | 3 (1.5) | 0 (0) |  | 3 (1.6) | 0 (0) |  | 2 (2.0) | 1 (0.9) |  |
T | T1 | 5 (5) | 4 (3.4) | 1 (1.0) | 0.236c | 5 (2.4) | 0 (0) | 0.057c | 5 (2.7) | 0 (0) | 0.808c | 4 (4.1) | 1 (0.9) | 0.724c |
 | T2 | 35 (35) | 15 (12.7) | 20 (20.8) |  | 35 (17.1) | 0 (0) |  | 29 (15.6) | 6 (23.1) |  | 13 (13.3) | 22 (19.3) |  |
 | T3 | 167 (166) | 93 (78.8) | 74 (77.1) |  | 159 (77.6) | 8 (88.9) |  | 147 (79.0) | 19 (73.1) |  | 77 (78.6) | 89 (78.1) |  |
 | T4 | 5 (4) | 4 (3.4) | 1 (1.0) |  | 4 (2.0) | 1 (11.1) |  | 3 (1.6) | 1 (3.8) |  | 2 (2.0) | 2 (1.8) |  |
 | missing | 2 (2) | 2 (1.7) | 0 (0) |  | 2 (1.0) | 0 (0) |  | 2 (1.1) | 0 (0) |  | 2 (2.0) | 0 (0) |  |
N | N(−) | 115 (114) | 70 (59.3) | 45 (46.9) | 0.050 a | 113 (55.1) | 2 (22.2) | 0.083b | 99 (53.2) | 15 (57.7) | 0.710a | 61 (62.2) | 53 (46.5) | 0.013 a |
 | N(+) | 97 (96) | 46 (39.0) | 51 (53.1) |  | 90 (43.9) | 7 (77.8) |  | 85 (45.7) | 11 (42.3) |  | 35 (35.7) | 61 (53.5) |  |
 | missing | 2 (2) | 2 (1.7) | 0 (0) |  | 2 (1.0) | 0 (0) |  | 2 (1.1) | 0 (0) |  | 2 (2.0) | 0 (0) |  |
Metastases | M(−) | 163 (161) | 88 (74.6) | 75 (78.1) | 0.367a | 159 (77.6) | 4 (44.4) | 0.037 b | 144 (77.4) | 17 (65.4) | 1.808a | 77 (78.6) | 84 (73.7) | 0.689a |
 | M(+) | 51 (51) | 30 (25.4) | 21 (21.9) |  | 46 (22.4) | 5 (55.6) |  | 42 (22.6) | 9 (34.6) |  | 21 (21.4) | 30 (26.3) |  |
Synchronous metastases | M(−) | 193 (191) | 104 (88.1) | 89 (92.7) | 0.224a | 188 (91.7) | 5 (55.6) | 0.004 b | 166 (89.2) | 25 (96.2) | 0.479b | 89 (90.8) | 102 (89.5) | 0.708a |
 | M(+) | 19 (19) | 13 (11.0) | 6 (6.3) |  | 15 (7.3) | 4 (44.4) |  | 18 (9.7) | 1 (3.8) |  | 8 (8.2) | 11 (9.6) |  |
 | missing | 2 (2) | 1 (0.8) | 1 (1.0) |  | 2 (1.0) | 0 (0) |  | 2 (1.1) | 0 (0) |  | 1 (1.0) | 1 (1.0) |  |
Metachronous metastases | M(−) | 176 (174) | 95 (80.5) | 81 (84.4) | 0.462a | 171 (83.4) | 5 (55.6) | 0.055b | 156 (83.9) | 18 (69.2) | 0.097b | 82 (83.7) | 92 (80.7) | 0.574a |
 | M(+) | 38 (38) | 23 (19.5) | 15 (15.6) |  | 34 (16.6) | 4 (44.4) |  | 30 (16.1) | 8 (30.8) |  | 16 (16.3) | 22 (19.3) |  |