Figure 6
From: Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells
Gal-7 and its R74S variant suppress PARP-1 cleavage, p53 expression and p21 transcription induced by doxorubicin. (A) Stable transfectants of MCF-7 cells expressing wild type or R74S mutant gal-7 were treated with 50 nM doxorubicin for 72 hrs at 37°C and immunoblotted with cleaved PARP-1-specific antibody. (B) Western blot analysis showing p53, p21 and gal-7 protein expression following doxorubicin treatment for 24 hrs. (C) mRNA levels of p53 and p21 were assayed by semi-quantitative RT-PCR. β-actin and GAPDH were used as loading controls.