Figure 7

BORIS and Sp1 interact with the TATA binding protein (hTBP). The methionine labeled “prey” fusion proteins Sp1, hTBP and the carboxyl terminus of hTBP (hTBP-C) were incubated with various resin-bound “bait” proteins as indicated below the polyacrylamide gels. The protein complexes were collected by precipitation and separated by gel electrophoresis. The weak band in the TNT-mix (negative control) is unspecific and serves as a background level (A). Schematic view of the detected protein-protein interactions. N, amino terminus; C, carboxyl terminus (B).