Figure 1
Comparison of the MAGE-A1 mRNA expression in 5-aza-CdR- and/or TSA-stimulated MCF-7 and BCM1 cells with the expression in BORIS-transfected cells. RT-PCR products of MAGE-A1 mRNA expression prior and after stimulation of MCF-7 (A) and BCM1 cells (B) with the demethylating agent 5-aza-CdR and/or the histone deacetylase inhibitor TSA or after transient transfection of these cells with an expression plasmid encoding for BORIS were separated on an agarose gel. The bar chart shows the relative changes in mRNA expression levels of MAGE-A1 in MCF-7 cells by quantitative real-time PCR. The significant p-values are shown (C). H2O lane serves as a negative control. The housekeeping gene β-Actin was selected as an internal control due to the lack of influence of any stimulation involved.