Figure 3

Gene transfection of STAT1C significantly decreases cell growth and tumorigenecity in ESCC cell lines. Using Western blot analysis, the gene transfection of STAT1C in EC1 and EC109 cells was shown to be effective, since the levels of STAT1, phospho-STAT1 and FLAG were dramatically increased 2 days after STAT1C transfection (A). Cell growth, as assessed by trypan blue cell counting, was found to be significantly decreased after STAT1C transfection in EC1 and EC109 cells (B) (* p < 0.05). Tumorigenecity, assessed by using colony formation assay, was significantly lower in EC1 and EC109 cells transfected with STAT1C, as compared to cells transfected with an empty vector (C) (** p < 0.001). (D) Transwell invasion assay showed that the transfection of STAT1C significantly inhibited cell invasion both ESCC cell lines. Results shown are representative of three independent experiments. (E.V.: empty vector).