FTY720 inhibits proliferation and metastasis of CC
. (A) Photomicrographs of xenograft tumors in nude mice. Representative images of a mouse in each group are presented. Tumor volumes in FTY720-treated mice were smaller than those of control mice. (B) The multiple tumor masses formed by the HuCCT1 cells in the FTY720-treated group were much smaller than those formed by HuCCT1 cells in the control group. (C) Tumors from different groups were immunostained for indicated molecules. CD31-stained microvessels were counted to record microvessel density. Apoptotic cells were counted to give the apoptosis index and cells expressing Ki-67 were counted to calculate the proliferation index. Pictures are representative of three independent experiments. (D) Western blot detection of the indicated molecules in tumor samples. β-Actin was used as the internal control. All assays were performed in triplicate. The results are expressed as the mean ± SD of three independent experiments; ***P <0.0001.