FTY720 inhibits the invasive potential of CC cells
. (A) FTY720 significantly inhibited the invasive capacity of QBC939, TFK-1 and HuCCT1 cells. ***P <0.0001. The results are presented as the mean ± SD of experiments performed in triplicate. (B) Western blot showing that FTY720 increased the expression of E-cadherin and decreased the expression of N-cadherin, vimentin, VEGF and TWIST1 in CC cells. β-Actin was used as the internal control. All assays were performed in triplicate. (C) Single and merged images show immunofluorescence staining of N-cadherin (green) and vimentin (red). The cell nucleus is stained blue by DAPI.