CXCL1 co-localizes with α-SMA and FSP1 positive stroma. Patient samples of breast ductal carcinoma were co-immunofluorescence stained for expression of CXCL1 (green) and α-SMA or FSP1 (red). Representative samples of CXCL1, α-SMA and FSP1 are shown. Sections were counterstained with DAPI. Secondary antibody only controls are shown: anti-goat-alexa-488 for CXCL1, anti-mouse-alexa-568 for α-SMA and anti-rabbit-alexa-568 for FSP1. Arrows and inset point to positive staining in fibroblastic cells. Scale bar =100 microns.